Notre Dame Integrated Imaging Facility

DAPI Staining

 DAPI Staining Protocol

Prepare the following solutions:

(To determine the amount needed for your experiment, please note that one tube of Solution 1 will yield a total of 100mL of working solution, at a final concentration of 0.5ug/mL.)

Solution 1*: 5 μL DAPI (10 mg/ml solution)

995 μL PBS

* Solution is viable for one month if stored at 4° in the dark

Solution 2 (working solution): 10 μL of Solution 1

990 μL PBS

Treatment: →Add Solution 2 (working solution) to your tissue/cells and incubate at room temperature for 1- 5 minutes.

→Rinse 2X with PBS.

→Mount coverslip with Shur/Mount, Prolong or comparable antifade medium.

→Seal coverslips (if necessary) with Valap or nail polish.**

**Try to avoid nail polish if are visualizing GFP, as the additives in nail polish tend to quench the signal.