Notre Dame Integrated Imaging Facility

IV Instruments and Services

The In Vivo Imaging Facility provides a non-invasive approach to observe various disease and biological models in living systems.  Our state-of-the-art instrumentation enables the detection of optical or nuclear signals to facilitate a number of applications.  We can detect luminescent genetic reporters (luciferase, GFP, RFP, β-gal, etc.) and fluorescent probes ranging from green to near infrared. We also offer planar X-ray or X-ray CT (3D) for digital radiographic imaging of various types of specimens.  Finally, we can detect radioactive PET and SPECT probes for  tomographic nuclear imaging of a number of disease states.

  Our goal is to train and empower users to independently utilize the facility and its resources.  Contact the Managing Director, Dr. W. Matthew Leevy (, for training, consultation, and/or experimental service.


The In Vivo Facility has upgraded all of our imaging equipment with safer isoflurane evacuation systems.  These EVAC4 waste gas evacuation systems provide the following features:

          -Simple and user friendly with a compact design.

          -Generates a 45 LPM negative flow that can be attenuated to appropriate levels and divided between three anesthesia stations not including an induction chamber.

          -Has a direct vacuum port that can be used with a Vapor-Vac Hooded Induction Chamber in conjunction with one other anesthesia station.

          -Adjustable negative flow meters easily activated with toggle switches.

          -Waste anesthetic gases can be discharged into activated charcoal canister or non-recirculating hood or vent.

          -Available without negative flow generating pump- can then be used to attenuate active, in-house vacuum source.

Each piece of imaging equipment has been set up to these new EVAC4 systems and connected to our in-house vacuum system.  The user must simply turn on the vacuum system at the spicket at the wall near the imaging station to activate the system and begin suction.  The flowmeters for the instrument and incubation chamber lines have already been set and adjusted on the EVAC4 system and will provide the most efficient flow rate to eliminate excess/waste isoflurane.  These flowmeter nozzles do not need to be adjusted but the toggle switches on the front panel of the EVAC4 must be in the "on" position for the system to operate correctly.  Once the user is done imaging, the vacuum spicket near the wall simply needs to be turned off to discontinue suction.  The EVAC4 systems will eliminate the use of F/air canisters and the possibility that the canisters in use are dated or are filled beyond maximum absorbance capacity.  We feel that these systems are necessary to our facility in order to provide our users the safest imaging environment as possible.  Please contact Sarah Chapman ( if you have any questions or concerns using the new EVAC4 systems.  Or click on the link to view more detailed instructions for usage and to gain more information about these systems.