Imaging Procedure for Infection Imaging
After an infection incubation time (usually 6 h), the mice will again be anesthetized and receive a tail vein injection of fluorescent or radioisotopic probe in water (75 HL). At times 0, 3, 6, 12, 18, and 24 h, post probe injection, the anesthetized mouse will be placed in the Imaging Station for a period of time generally no longer than 10 minutes. Anesthesia will be maintained with nose cones. After each imaging acquisition, the animals will be allowed to recover from the anesthesia and returned to their cohort in the housing facility. After each group of animals is imaged, the area will be wiped with 70% ethanol or clidox. After the final image acquisition at 24 h, the mice will be euthanized while under anesthesia (isofluorane) by cervical dislocation. Dissection will be performed to confirm that the probes are localized at the infection site.