IVIS Lumina, Bruker MSFX and Xtreme Imaging Station
The imaging studies employ a Kodak Multispectral Image Station FX and/or Xtreme Image Station, which are capable of imaging in four modalities: bioluminescence, fluorescence, X-ray, and radioisotopic. These studies will be performed in the Notre Dame Integrated Imaging Facility. The image station is equipped with built-in nose cones that deliver isoflurane. The anesthetic depth is monitored by checking mouse breathing once every minute by visual inspection. The anesthetic dose will be lowered for animals that are observed to be struggling for a normal breathing pattern. The anesthetic dose will be raised for animals that retract their feet after a firm toe pinch.
The isoflurane inside the Imaging Station is vented through a hose that forces the isoflurane through a commercial scavenging filter that is monitored for weight gain.
The X-ray power is 35 kVp and delivers an approximate dose of 1 rad, which is well below the safety threshold of 100 rad.
The above SOP can be used for the IVIS Lumina with the exception of the X-ray capability.