Notre Dame Integrated Imaging Facility

GE Healthcare DeltaVision Deconvolution Microscope

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The DeltaVision imaging system is an inverted microscope that allows the user to capture widefield epi-fluorescence images, and with the deconvolution algorithm redirect all out of focus light to become in focus for sharp images.  It can do this with fixed cells and/or tissues, but its greatest strength is its ability to obtain and analyze long-term, time-lapse, three-dimensional images with a minimum of photo-damage to live cells or small organisms.  It can even track moving cells over time.  The system is equipped with an environmental chamber that allows a researcher to control temperature and carbon dioxide levels during runs, and with the sophisticated softWoRx image analysis and model building software the system provides a comprehensive package for biological image data collection, interpretation, deconvolution, and display.  This system has the following objective lenses to choose from; 40x oil, 60x oil, and 100x oil.  There are two sets of filters that the user can choose, the standard set for fixed cells with excitation and emission ranges for DAPI, FITC, TRITC, and Cy5 or the live cell set with the wavelength ranges for CFP, YFP, GFP and mCherry.  One can also choose between two available cameras to acquire the images, a CoolSnap HQ2 for high resolution images or an EMCCD Cascade II/512 high-sensitivity camera for samples with low light.